Gene Researcher at the Taubert Lab: Day 4 & 5

18 03 2012

I spent my last two days at the Taubert lab extracting plasmids from bacteria. I followed the alkaline lysis procedure in which bacteria is exposed to NAOH and sodium dodecyl sulfate (SDS), a strong detergent. The cell membranes of the bacteria burst and the substances inside the bacteria spill out. Sodium acetate, an acidic solution, is then added to neutralize the solution. I observed that by this point, the cell membrane material and DNA material have precipitated. I then separate the cell contents by centrifugation. The resulting substance is extracted to purify the plasmid DNA.

I used a spectrophotometry too. It is a photometer that can measure intensity as a function of the light source wavelength. It is used commonly used for the measurement of transmittance or reflectance of solutions, but it can also be used to measure the equilibrium constant of a solution.

I have learned so much this week at the lab. I can’t wait to work in a genetics lab in the future!!

– Alice Yip

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2 responses

18 03 2012
valeryzaby

” I can’t wait to work in a genetics lab in the future “. You’re going to become a mini Catherine :)))) I can’t wait until you make epic genetics discoveries ~

19 03 2012
megannantel

It`s so cool that you`ve found something you really like!! Genetics sounds pretty complicated to me!! :/

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